Applejack – honesty
Applejack possesses the element of honesty.
The first element to be demonstrated during the journey. When a mountain ledge the friends are standing on crumbles, Twilight clings onto a ledge and is bewildered when Applejack tells her to let go because she is safe. Twilight trusts her and lets go. Rainbow Dash and Fluttershy catch her mid-fall and carry her to safety.
The element of honesty is as a gold necklace with an orange apple-shaped gem that looks like the apples in Applejack's cutie mark.
Fluttershy – kindness
Fluttershy possesses the element of kindness.
When the friends encounter an angry manticore, they decide to fight it, but Fluttershy calmly approaches it. She removes a thorn stuck in the manticore's paw, calming the beast.The element of kindness is as a gold necklace with a pink butterfly-shaped gem that looks like Fluttershy's cutie mark.
Pinkie Pie – laughter
Pinkie Pie possesses the element of laughter.
The friends enter a dense forest of trees with scary faces, but Pinkie Pie laughs and makes funny faces at the trees, causing the faces on the trees to vanish in pink smoke with sparkles. She explains that they can conquer their fears just by laughing at them.
The element of laughter is a gold necklace with a sky blue balloon-shaped gem that looks like Pinkie's cutie mark.
Rarity – generosity
Rarity possesses the element of generosity.
The friends cannot cross a river because a sea serpent, who is upset about his ruined mustache, is agitating the water. Rarity sympathizes with him and cuts off her own tail to repair his mustache. The grateful sea serpent helps them cross the river.
The element of generosity is as a gold necklace with a purple diamond-shaped gem that looks like Rarity's cutie mark. The necklace itself is also similar to Princess Celestia's necklace.
Rainbow Dash – loyalty
Rainbow Dash possesses the element of loyalty.
The ponies encounter a broken rope bridge stretching across a ravine. Rainbow Dash volunteers to fly over and tie the other end of the bridge, but is confronted by a group of dark ponies who call themselves The Shadowbolts. They flatter Rainbow Dash and invite her to join them as their captain, as long as she abandons her friends. Rainbow Dash is tempted, but ultimately declines the Shadowbolts' offer. She ties the bridge, letting the others cross.
The element of loyalty is as a gold necklace with a red thunderbolt-shaped gem, shaped similarly to Rainbow Dash's cutie mark.
Twilight Sparkle – magic
Twilight Sparkle possesses the element of magic.
When her friends come to her aid in the castle ruins, she realizes that they are her friends, and a "spark" within her activates the Elements of Harmony.
The element of magic is a gold tiara studded with sapphires and a magenta star-shaped gem that looks like Twilight's cutie mark. After her transformation into a alicorn, her tiara has a few changes: it has become a lighter shade of gold, the sapphires are removed and the magenta gem is lowered a bit down.